LB 89, aka the "Stand With Women Act" ensures that males are not competing in female sports.
More than two dozen states have similar laws in place.
Nate Grasz of Nebraska Family Alliance says it's just common sense.
"We are thankful that after years of work on this issue, Nebraska has now successfully passed legislation to keep girls sports for girls and ensure fairness, safety, and equal opportunity in girls sports," says Grasz, executive director of Nebraska Family Alliance.
Bills in Nebraska have to go through three separate rounds of debate.
Grasz says the opposition did not go down without a fight.
"LB 89 had to overcome multiple filibusters," says Grasz. "33 votes were needed to advance the bill and thankfully we had 33 votes exactly to pass this into law, so this was a hard-fought win but it's a victory not only for the protection of women and girls but for truth itself."
President Donald Trump (right) signed an executive order banning biological males in girls sports in the early days of his second term. Not all states are in compliance with the EO.
The bill defines “male” and “female” and requires students to participate in sports based on their biological sex.
The bill includes a requirement for a physical and a doctor’s note verifying sex at birth before participation in sports.

It defines sex based on the person’s reproductive system.
Opponents argue the bill is politically motivated and targets transgender individuals, particularly children, while misinterpreting federal Title IX protections.
The bill will impact all 245 public school districts, community colleges, state colleges and the University of Nebraska.
Grasz says Governor Jim Pillen (R-Nebraska) will sign the measure into law next week.
"Governor Pillen has been a vocal supporter of protecting girl sports and will be signing LB 89 into law next week," says Grasz.
The bill expands portions of the Women’s Bill of Rights, which Pillen established via executive order in August 2023, to schools, sports teams and state facilities.
Pillen actually requested that the legislature take this on.
University of Nebraska softball player Jordy Bahl commended the courage of public officials willing to support LB89.
“Taking a stance on this issue is a no brainer for me. Many more people feel the same way on this issue, but after experiencing the hate one receives for taking this stand, I understand why they don’t all come forward,” she said. “That will never stop me from using my voice to defend the safety of women. Not just in sports, but in all women-designated spaces.”